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Figure 10.31. Identi cation of phosphopeptides by solid phase capture. Phosphopeptides in a tryptic digest are blocked with tBOC groups followed by condensation of phosphates/carboxyls to ethanolamine. Tri uoroacetic acid (TFA) regenerates phosphates selectively and these are reacted with cystamine introducing SH on phosphates. Phosphopeptides are captured by iodoacetic acid coated glass beads. TFA releases and deblocks peptides which are analysed by LC-Tandem MS

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Headquarters Few learned paths between HQ and manufacturing plant: 1. A-C-E-F 2. A-B-E-F 3. A-C-D-F 4. A-C-B-E-F 5. A-B-C-D-E-F

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If the Options bar is hidden or you just don t like reaching to click the button, you can commit text by selecting any other tool, clicking any palette but the Character or Paragraph palette, or pressing Ctrl+Enter. While you re in text edit mode, most menu commands are unavailable. You must commit the text or cancel the current type operation to regain access to them. To abandon your type operation, click the Cancel button the large X at the right end of the Options bar or press Esc. When you create the first bit of type in an image, Photoshop creates a new layer to hold the text. After you commit the type, clicking or dragging with the type tool has one of two outcomes. If Photoshop finds any text near the spot where you click or drag, it assumes that you want to edit that text and, therefore, selects the text layer and puts the type tool into edit mode. For paragraph text, the paragraph is selected as well. If no text is in the vicinity of the spot you click, the program decides that you must want to create a brand new text layer, and responds accordingly. You can force Photoshop to take this second route by Shift-clicking or Shift-dragging with the type tool, which comes in handy if you want to create one block of text on top of another.

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If you want to share an Internet connection from a Windows XP SP2 machine with other computers on a local network, you must use the Network Setup Wizard to share that connection with those other machines. To do this, click Start My Network Places and then select the entry that reads Set up a home or small office network under the Network Tasks heading in that window. As you work with the Network Setup Wizard, it guides you through a number of steps involved in setting up a shared Internet connection through your XP machine. By default, however, this wizard configures the gateway machine to respond to incoming pings from the Internet (Start Control Panel Security Center Windows Firewall, click the Advanced tab, and then click the Settings button in the ICMP pane) as shown in first sidebar figure. This setting is actually worded Allow incoming echo request, but for all practical purposes that means respond to incoming pings.

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New Feature Quickstart links are new for AutoCAD 2006 and AutoCAD LT 2006. Some other locations for these links are on a new tool palette and on the Sheet Set Manager (both discussed in 26).

2.1.4.7 2.1.4.8 2.1.4.9 2.1.4.10 2.1.4.11

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