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When generating calibration equations using samples of known composition, the independent variable is represented by the spectroscopic readings (ie, log 1/R) at speci c wavelengths, while the concentration of the analyte of interest, for example, determined by traditional laboratory techniques, is the dependent variable Spectral raw data may have a distribution or shape that is not optimal for analysis Background effects, baseline shifts, measurements in different conditions, different variances in interfering variables and the like can complicate information extraction using multivariate methods It is important to minimize the noise introduced by such effects Preprocessing operations include centering, weighting, and numerous mathematical transformations Mean centering consists of subtracting the average spectra from each individual spectrum This ensures that all results will be interpretable in terms of variation around the mean considered as the model center.

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This is useful with models in which a linear relationship between spectral data and response data is supposed to go through the origin Depending on the kind of information to be extracted from the spectral data, weights based on the standard deviation (ie, square root of the variance, which expresses the variance in the same unit as the original variable) may be used for scaling This may be a typical pretreatment for PCA, PLS, or PCR calibrations which are projection methods based on nding directions of maximum variation, thus depending on the relative variance of the variables A possible weighting option is the 1/SD standardization, which gives all variables the same variance, that is, 1 In this case all variables are given the same chance to in uence estimation of the components.

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This is advisable if the variables are measured with different units, have different ranges, or are of different types It is also possible to x a constant weight for each variable manually Weighting involves stretching and shrinking by measuring a position relative to the extremes in the actual spectral data table By standardizing the spectra, variation in the data set is performed relative to the extremes in the data table However, this procedure emphasizes the relative in uence of unreliable or noisy attributes 4236 Mathematical Pretreatment and Transformation.

Figure 1.1b Andre Duerinckx, the Conference Chairman of the First SPIE Medical Imaging Conference (International Society for Optical Engineering) where the term PACS was coined.

the Internet under most circumstances. Ports 1028 and 4500 are temporary UDP ports used for file transfer with another machine on my local network. Again, they needn t be open for general Internet access unless you re running some kind of application that warrants their presence. Port 123 is associated with the Network Time Protocol, used to synchronize my local system clocks with the time server at Texas A&M University; if you don t use a time service you shouldn t see this active, either. Finally, ports 1036 and 1039 are associated with specific IP addresses at Webroot.com, in conjunction with my use of the Spy Sweeper software. In this case, open ports were purely local with no Internet access, except for 1036 and 1039 as noted.

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of concentric circular fringes For the light source we can use a sodium or even a uorescent lamp In the situation where the laser is the source of light, there is a much higher limit for the value of t Even though several meters can be used for t, we shall set t 1000 mm In this case, using l 632:8 nm, we get for f an upper limit of 5 s of arc Hence it is not dif cult to design a collimating system to satisfy this condition Another aspect that is important, especially with large values of t, is the lateral shear one can get in the instrument To avoid this, the autocollimated pinhole images must coincide with the pinhole itself Similarly, if the collimating lens is not properly collimated, either a convergent or a divergent beam will emerge.

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